Induction of acetoacetate decarboxylase in Clostridium acetobutylicum
نویسندگان
چکیده
منابع مشابه
Cloning, sequencing, and molecular analysis of the acetoacetate decarboxylase gene region from Clostridium acetobutylicum.
Acetoacetate decarboxylase (ADC) (EC4.1.1.4) of Clostridium acetobutylicum DSM 792 was purified to homogeneity, and its first 25 N-terminal amino acids were determined. Oligonucleotide probes deduced from this sequence were used to detect positive clones in partial gene banks derived from Sau3A and HaeIII digests with following ligation into the vector pUC9. In Escherichia coli, the 2.1-kbp Hae...
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Clostridium acetobutylicum NRRL B527 and ATCC 824 exhibited extracellular and cell-bound endoglucanase and cellobiase activities during growth in a chemically defined medium with cellobiose as the sole source of carbohydrate. For both strains, the endoglucanase was found to be mainly extracellular (70 to 90%) during growth in continuous or batch cultures with the pH maintained at 5.2, whereas t...
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The purification and concentration of enzymes by adsorption on charcoal, tannin, aluminum hydroxide, ion exchange resins, and so forth, has been an established practice in the laboratory. Some years before this work was done in 1949, alkali-cooked lignin was used as an adsorbent for mold and malt amylase (Wallerstein et al., 1945). In this paper is described our study of the adsorption of the a...
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Crude extracts of Clostridium acetobutylicum contain a butyrate kinase of high specific activity (5.2 mumol/min/mg of protein). The enzyme has been purified 77-fold in a six-step procedure to a specific activity of 402 mumol/min/mg of protein. The purified butyrate kinase showed a single band with a molecular weight of 85,000 on nondenaturing polyacrylamide gradient gel electrophoresis. Separat...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1985
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1985.tb00875.x